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Related Experiment Video

Updated: Jun 27, 2026

Primary Culture of Adult Rat Heart Myocytes

Primary Culture of Adult Rat Heart Myocytes

Published on: June 16, 2009

K(ATP) channels are involved in regulatory volume decrease in rat cardiac myocytes.

L Shi¹, M Xu, J Liu

¹Department of Physiology, School of Basic Medical Sciences, Department of Respiratory Medicine, Capital Medical University, Beijing 100069, China.

Physiological Research

|December 20, 2008

Summary

Regulatory volume decrease (RVD) in rat ventricular myocytes relies on K(ATP) channels. These channels, specifically Kir6.1 and Kir6.2, are crucial for cell volume recovery following hypotonic stress.

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Published on: September 14, 2012

Area of Science:

Cellular physiology
Ion channel function
Cardiovascular research

Background:

Regulatory volume decrease (RVD) is vital for animal cell survival.
Understanding RVD mechanisms in cardiac cells is crucial for cardiovascular health.

Purpose of the Study:

To investigate the RVD process in rat ventricular myocytes.
To determine the involvement of K(ATP) channels in this RVD process.

Main Methods:

Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis to identify K(ATP) channel subtypes.
Exposure of myocytes to hypotonic solutions to induce RVD.
Pharmacological inhibition of ion channels (Cl(-), K(+), K(ATP)) and electrophysiological recordings.

Main Results:

Rat ventricular myocytes express Kir6.1 and Kir6.2 K(ATP) channels.
Hypotonic stress induced RVD, characterized by initial swelling and subsequent volume recovery.
RVD was inhibited by 9-AC (Cl(-) channel blocker), CsCl (K(+) channel blocker), and glibenclamide (K(ATP) channel blocker).
Electrophysiology revealed hypotonic solution activated a glibenclamide-sensitive current resembling K(ATP) channel activity.

Conclusions:

The RVD process in rat ventricular myocytes is dependent on K(+) channel activation.
K(ATP) channels play a significant role in mediating RVD in these cardiac cells.