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A comparative study of the 'FDA' and 'USDA' methods for the detection of Listeria monocytogenes in foods
D W Warburton1, J M Farber, A Armstrong
1Microbiology Evaluation Division Bureau of Microbial Hazards, Health and Welfare Canada, Ottawa, Ontario.
Abstract:
Nineteen laboratories across Canada took part in a comparative study of the 'FDA' and 'USDA' methods for the detection of Listeria monocytogenes in foods and environmental samples. The results show that the enrichment period of the FDA method can be shortened from 7 to 2 days without substantially reducing the number of positive samples. With a limited number of samples, the USDA method proved to be slightly more efficient in isolating L. monocytogenes than the FDA method. Fraser broth, in principle, proved to be useful as a screening tool but is not very selective. Oxford agar and lithium chloride-phenylethanol-moxalactam medium were better than modified McBride's agar in isolating this microorganism.