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Related Experiment Video

Updated: Jun 27, 2026

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Published on: August 17, 2022

Hepatocyte transplantation techniques: large animal models.

Anne Weber1, Marie-Thérèse Groyer-Picard, Ibrahim Dagher

  • 1Hôpital de Bicêtre, Inserm U 804; University Paris-Sud, Clamart, Kremlin-Bicêtre, France.

Methods in Molecular Biology (Clifton, N.J.)
|December 20, 2008
PubMed
Summary

Hepatocyte transplantation for liver disease is limited by poor cell engraftment. Partial portal vein embolization in primates stimulates transplanted hepatocyte proliferation, offering a translatable clinical strategy.

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Area of Science:

  • Hepatology
  • Regenerative Medicine
  • Cell Therapy

Background:

  • Hepatocyte engraftment and expansion are critical for cell therapy success in liver diseases.
  • Current rodent models for liver repopulation are not clinically translatable.
  • Safe and effective methods for promoting transplanted hepatocyte proliferation in humans are lacking.

Purpose of the Study:

  • To define strategies in large animal models that stimulate transplanted hepatocyte proliferation for clinical translation.
  • To establish a preclinical model for evaluating cell therapy approaches in liver disease.

Main Methods:

  • Developed a protocol involving partial portal vein embolization in non-human primates (Macaca mulatta).
  • Assessed the proliferation of transplanted hepatocytes following embolization.

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Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
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Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Published on: November 4, 2018

Related Experiment Videos

Last Updated: Jun 27, 2026

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Published on: August 17, 2022

Orthotopic Liver Transplantation in Rats
18:25

Orthotopic Liver Transplantation in Rats

Published on: July 1, 2012

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
09:54

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Published on: November 4, 2018

  • Evaluated the suitability of this model for lentiviral-mediated ex vivo gene therapy.

    • Main Results:

    • Partial portal vein embolization successfully induced proliferation of transplanted hepatocytes in non-human primates.
    • Simian hepatocytes demonstrated efficient transduction by lentiviral vectors.
    • This model supports the evaluation of cell and gene therapy strategies for liver diseases.

    Conclusions:

    • Partial portal vein embolization is a promising strategy to enhance hepatocyte proliferation for cell therapy.
    • Non-human primates serve as a relevant model for developing translatable liver cell therapies.
    • The model is suitable for assessing lentiviral gene therapy approaches in conjunction with cell transplantation.