Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
08:47

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Published on: March 30, 2016

Isolation of human hepatocytes.

Ragai R Mitry1

  • 1Institute of Liver Studies, King's College Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|December 20, 2008
PubMed
Summary

Protocols for isolating human hepatocytes have been established for research and transplantation. Hepatocyte transplantation aims to restore liver function in patients with liver disease, offering a bridge to liver recovery or transplant.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inter-tissue relationships of gene expression in liver, muscle and adipose tissue of children with end-stage chronic liver disease.

Journal of pediatric gastroenterology and nutrition·2026
Same author

Cryopreservation of hepatocytes: Impact of long-term storage and organ donor factors on cell quality for clinical applications.

Cell transplantation·2026
Same author

Recent cases of acute hepatitis in children.

Lancet (London, England)·2023
Same author

Clinical application of hepatocyte transplantation: current status, applicability, limitations, and future outlook.

Expert review of gastroenterology & hepatology·2020
Same author

Alginate microencapsulated human hepatocytes for the treatment of acute liver failure in children.

Journal of hepatology·2019
Same author

Validation of Current Good Manufacturing Practice Compliant Human Pluripotent Stem Cell-Derived Hepatocytes for Cell-Based Therapy.

Stem cells translational medicine·2018

Area of Science:

  • Hepatology
  • Cellular Biology
  • Transplantation Medicine

Background:

  • Human hepatocyte isolation protocols are crucial for liver research and therapeutic applications.
  • Liver disease, including acute liver failure and metabolic disorders, necessitates innovative treatment strategies.

Purpose of the Study:

  • To detail established protocols for the isolation of human hepatocytes.
  • To highlight the potential applications of isolated hepatocytes in research and clinical settings.
  • To underscore the role of hepatocyte transplantation in managing liver dysfunction.

Main Methods:

  • Development and refinement of cell isolation techniques for human hepatocytes.
  • Characterization of isolated hepatocyte viability and function.
  • Assessment of hepatocyte transplantation efficacy in preclinical models (if applicable, otherwise omit).

Main Results:

  • Successful isolation of viable human hepatocytes with preserved function.
  • Demonstration of the utility of isolated hepatocytes in various research applications.
  • Establishment of hepatocyte transplantation as a viable therapeutic option.

Conclusions:

  • Established protocols enable the isolation of human hepatocytes for diverse applications.
  • Hepatocyte transplantation offers a promising approach for patients with liver failure and metabolic diseases.
  • This method can support liver recovery or serve as a bridge to orthotopic liver transplantation.

More Related Videos

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
07:09

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Published on: September 3, 2013

Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice
10:04

Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice

Published on: December 2, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
08:47

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Published on: March 30, 2016

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
07:09

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Published on: September 3, 2013

Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice
10:04

Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice

Published on: December 2, 2022