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Related Experiment Video

Updated: Jun 27, 2026

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
07:14

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

A new procalcitonin optical immunosensor for POCT applications.

F Baldini1, L Bolzoni, A Giannetti

  • 1Institute of Applied Physics, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy. f.baldini@ifac.cnr.it

Analytical and Bioanalytical Chemistry
|December 20, 2008
PubMed
Summary

A novel immunosensor for procalcitonin detection was created using a polymethylmetacrylate optical biochip. This point-of-care test offers sensitive detection in buffer and human serum samples.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Immunotechnology

Background:

  • Procalcitonin (PCT) is a biomarker for bacterial infections and sepsis.
  • Accurate and rapid PCT determination is crucial for timely clinical decisions.
  • Existing diagnostic methods may lack the speed or sensitivity required for point-of-care settings.

Purpose of the Study:

  • To develop a novel immunosensor for sensitive procalcitonin (PCT) determination.
  • To implement a sandwich assay on an optical biochip for potential point-of-care testing (POCT).
  • To evaluate the assay's performance in both buffer and human serum samples.

Main Methods:

  • Development of a sandwich immunoassay using two rat monoclonal antibodies.
  • Immobilization of a capture antibody onto a polymethylmetacrylate (PMMA) optical biochip with flow channels.
  • Labeling of the detection antibody with DY647 fluorescent dye and optimization to minimize non-specific adsorption.

Main Results:

  • Achieved a limit of detection (LOD) of 0.088 mg L⁻¹ within a working range of 0.28–50 mg L⁻¹ in buffer.
  • Successfully adapted the assay for human serum samples.
  • Attained an LOD of 0.2 mg L⁻¹ and a working range of 0.7–25 mg L⁻¹ in human serum.

Conclusions:

  • The developed immunosensor demonstrates high sensitivity and a suitable working range for procalcitonin detection.
  • The optical biochip platform is adaptable for point-of-care testing applications.
  • This technology offers a promising tool for rapid and accurate procalcitonin diagnostics in clinical settings.