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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
Biomaterial-induced sarcomagenesis is not associated with microsatellite instability
Achim Weber1, Annette Strehl, Erik Springer
1Institute of Pathology, Johannes Gutenberg University of Mainz, Langenbeckstr. 1, 55101, Mainz, Germany. achim.weber@usz.ch
Virchows Archiv : an International Journal of Pathology
|December 20, 2008
Summary
Biomaterial-induced sarcomas in rats did not show microsatellite instability, a common feature in many cancers. This suggests a mutator phenotype is not a key factor in the development of these specific malignant mesenchymal tumors.
Area of Science:
- Biomedical Science
- Oncology
- Materials Science
Background:
- Sarcomagenesis, the development of sarcomas, is less understood than carcinogenesis.
- Microsatellite instability (MSI) is linked to various cancers, especially those with chronic inflammation.
- Biomaterials can induce chronic inflammation and malignant mesenchymal tumors in animal models.
Purpose of the Study:
- To investigate if biomaterial-induced sarcomas exhibit a mutator phenotype.
- To determine the role of microsatellite instability in biomaterial-induced sarcomagenesis.
Main Methods:
- Utilized an experimental animal model (rats) exposed to biomaterials.
- Employed a multiplex-PCR approach to screen ten biomaterial-induced sarcomas for MSI.
- Analyzed seven distinct microsatellite loci using fluorochrome-labeled PCR and fragment analysis.
Main Results:
- All ten tested tumors displayed a microsatellite-stable (MSS) phenotype.
- No microsatellite instability was detected across any of the seven evaluated loci.
- The findings indicate MSI is not a characteristic of these specific tumors.
Conclusions:
- Biomaterial-induced sarcomagenesis in this model is not associated with a mutator phenotype.
- Microsatellite instability appears to play a minimal or no role in the malignant transformation of these soft tissue tumors.
- Further research is needed to elucidate the mechanisms driving biomaterial-induced sarcomagenesis.
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