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Eliminating heterophilic antibody interference for ferritin detection using Olympus F(ab')2 based reagent
Eric Grouzmann1, Nicole Dayer, Catherine Bain
1Laboratoires Meditest SA, 1800, Vevey, Switzerland. eric.grouzmann@meditest.ch
Clinical Laboratory
|December 23, 2008
Summary
Rabbit-derived antibodies in the Olympus ferritin assay can cause interference. This improved assay eliminates heterophilic antibody interference, ensuring accurate ferritin level measurements.
Area of Science:
- Clinical Chemistry
- Immunology
Background:
- The Olympus immunoturbidimetric assay for ferritin has been subject to interferences.
- These interferences are attributed to the use of rabbit-derived antibodies.
- Rabbits are known sources of heterophilic antibodies, which can disrupt immunoassays.
Purpose of the Study:
- To report on the improvement of the Olympus Ferritin assay.
- To eliminate interference from heterophilic antibodies in ferritin testing.
Main Methods:
- Modification of the Olympus immunoturbidimetric assay for ferritin.
- Characterization of antibody sources and potential for heterophilic antibody generation.
Main Results:
- The improved Olympus Ferritin assay effectively eliminates interference from heterophilic antibodies.
- Accurate ferritin measurements are restored after assay modification.
Conclusions:
- Assay modifications have successfully addressed heterophilic antibody interference.
- The enhanced Olympus Ferritin assay provides reliable ferritin quantification.
