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Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
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An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
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Published on: December 17, 2018

Membrane optode for mercury(II) determination in aqueous samples.

Y Kalyan1, A K Pandey, P R Bhagat

  • 1Department of Environmental Sciences, S.V. University, Tirupati 517502, India.

Journal of Hazardous Materials
|December 23, 2008
PubMed
Summary

A novel color-changing optode effectively detects mercury (Hg(II)) and methylmercury (CH3Hg+) in water. This reusable sensor offers a rapid and sensitive method for environmental mercury monitoring.

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A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
12:59

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry

Published on: January 8, 2016

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Materials Science

Background:

  • Mercury (Hg(II)) and methylmercury (CH3Hg+) pose significant environmental and health risks.
  • Accurate and sensitive detection methods are crucial for monitoring mercury contamination in aqueous samples.

Purpose of the Study:

  • To develop a reusable, color-changeable optode for the quantitative preconcentration and spectrophotometric determination of Hg(II) and CH3Hg+.
  • To investigate the optode's performance characteristics, including response time, selectivity, and sensitivity.

Main Methods:

  • Immobilization of 4-(2-pyridylazo)resorcinol (PAR) dye and Aliquat-336 liquid ion-exchanger in a cellulose triacetate matrix.
  • Spectrophotometric analysis of Hg(II) and CH3Hg+ preconcentrated on the optode.
  • Validation using radiotracer, EDXRF, and INAA techniques.

Main Results:

  • The optode exhibited a distinct color change (lambda(max)=520 nm) upon Hg(II) and CH3Hg+ binding within 5-30 minutes.
  • Hg(II) uptake was pH-dependent, with maximum efficiency (>90%) at pH 7.5.
  • The optode showed high selectivity for Hg(II) over other metal ions (Cu(II), Fe(II), Zn(II), Pb(II)), with minor interference from Cd(II).
  • Detection limits were as low as 1 microg (INAA) and a linear response was observed for Hg(II) concentrations from 0.22-1.32 microg/mL.
  • Preconcentration from large sample volumes extended the lower quantification limit to 0.11 microg/mL.

Conclusions:

  • The developed optode is a sensitive, selective, and reusable tool for detecting mercury species in aqueous samples.
  • The method offers a rapid and cost-effective alternative for environmental mercury monitoring.