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Published on: August 5, 2022
Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola
Atsushi Minami1, Naoto Tajima, Yusuke Higuchi
1Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan. aminami@mail.sci.hokudai.ac.jp
Abstract:
The biosynthetic gene cluster of brassicicene C was identified in Alternaria brassicicola strain ATCC 96836 from genome database search. In vivo and in vitro study clearly revealed the function of Orf8 and Orf6 as a fusicoccadiene synthase and methyltransferase, respectively. The understanding toward the biosynthetic pathway promises construction of this type of diterpene compounds with genetic engineering.
