Related Experiment Video
Updated: Jun 27, 2026
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
A sensitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of pharmaceutical
Dawei Li1, Shuang Wei, Hong Yang
1Key Laboratory of Green Chemistry & Technology, Ministry of Education of China, College of Chemistry, Sichuan University, Chengdu 610064, PR China.
Abstract:
One-step membrane-based competitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of indomethacin (IDM) in water samples was developed. A positive reaction as a result of the remaining gold-antibody conjugate combining with antigen coated on the membrane was obvious by visual detection. Under optimal conditions, the visual detection limit was found to be 0.1 ngmL(-1), which was about 10-1000 times lower than those in published literatures, demonstrating the high sensitivity of the assay. The stability test indicated the immunochromatographic strips could be used for 8 weeks at room temperature without significant loss of activity. Real water samples were detected by the assay and confirmed by enzyme-linked immunosorbent assay. The assay time for indomethacin detection was less than 10 min, suitable for rapid testing on-site.
More Related Videos
11:48Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
Published on: July 13, 2016
11:19Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
Published on: March 7, 2016
