Rapid enzymatic method for the measurement of mannitol in urine
Related Experiment Videos
J Blood1, A R Ingle, N Allison
1Department of Chemical Pathology, Derbyshire Royal Infirmary, Derby, UK.
Annals of Clinical Biochemistry
|July 1, 1991
Abstract:
A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or sugar alcohols could be demonstrated. The method is precise (within-batch CV less than 1%), rapid and shows excellent recovery of mannitol in spiked samples. Comparison with gas liquid chromatography shows excellent correlation (r = 0.994) between the two methods.