Optimum threonine requirement of laying hens

1. One experiment was conducted with medium weight laying hens to determine their threonine requirement between 28-38 weeks. 2. Two threonine-limiting diets of identical protein quality (summit-dilution) were used and, by dilution, ten protein contents were produced supplying 2.7 to 5.4 g total threonine/kg diet. The diet with the lowest protein was also supplemented with synthetic L-threonine. Each diet was fed to 5 groups of 24 laying hens. 3. The daily threonine requirement of the individual laying hens was estimated by direct methods to be 8.7 mg/g egg output plus 43.49 mg/kg body weight for this experiment. Calculated optimum intakes of threonine for various ratios of costs of input to value of output are tabulated. For example, for a flock of medium weight laying hens producing an average of 50 g daily egg mass, the optimum threonine intake (mg/hen d) varied between 700 and 710 for cost ratios (k-values) varying between 0.002 and 0.001.