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Colloidal precipitates

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Updated: Jun 27, 2026

Monitoring Protein Adsorption with Solid-state Nanopores

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Modulating colloidal adsorption on a two-dimensional protein crystal.

Matthew M Shindel¹, Ali Mohraz, Daniel R Mumm

¹Department of Chemical Engineering and Materials Science, University of California, Irvine, California 92697-2575, USA.

Langmuir : the ACS Journal of Surfaces and Colloids

|December 23, 2008

Summary

This summary is machine-generated.

Modulating streptavidin-nanoparticle interactions is key for bottom-up assembly. This study shows pH and biotinylation significantly impact gold nanoparticle adsorption onto streptavidin crystals, with biotinylation enhancing attachment.

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Monitoring Protein Adsorption with Solid-state Nanopores

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Published on: December 2, 2011

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Published on: March 28, 2011

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Area of Science:

Biophysics
Materials Science
Nanotechnology

Background:

Streptavidin's properties enable nanoscale construction.
Controlling protein-nanoparticle interactions is crucial for bottom-up assembly.
Understanding adsorption mechanisms is vital for designing nanoscale devices.

Purpose of the Study:

To investigate the influence of pH and biotin-streptavidin interactions on gold nanoparticle adsorption.
To determine how these factors affect surface coverage and adsorption kinetics.
To explore selective desorption strategies for nanoparticle manipulation.

Main Methods:

Deposition of colloidal gold nanoparticles (biotinylated and non-biotinylated) onto streptavidin crystals at varying pH (6, 7, 8).
Analysis of particle surface coverage as a function of deposition time and pH.
Application of Derjaguin-Landau-Verwey-Overbeek (DLVO) theory to model interparticle potentials.
Investigation of selective desorption via post-adsorption pH changes.

Main Results:

Particle surface coverage increased 1.4-10 times with biotinylated nanoparticles.
Highest surface coverage for both particle types occurred at pH 6, decreasing with increasing pH.
Electrostatic interactions, not interparticle repulsion, primarily drive surface coverage trends.
Electrostatically adsorbed particles showed selective desorption upon pH alteration.
Receptor-ligand adsorption (biotin-streptavidin) exhibited faster kinetics and higher coverage than electrostatic adsorption.

Conclusions:

pH and biotinylation are critical parameters for controlling nanoparticle adsorption on streptavidin.
Electrostatic interactions significantly influence particle-surface binding.
The biotin-streptavidin interaction provides a robust mechanism for directed nanoparticle assembly.
Selective desorption offers a method for manipulating nanoparticle arrangements on surfaces.