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Updated: Jun 27, 2026
A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
Published on: April 16, 2018
[Increased expression of transient receptor potential melastatin 7 in mouse cardiac fibroblasts post myocardial
Ming-jiang Li1, Yong-ming Zhou, Yan-hong Tang
1Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China. li_mingjiang@hotmail.com
Objective:
To observe transient receptor potential melastatin 7-like (TRPM7L) expression changes post myocardial infarction (MI) in mouse cardiac fibroblast (CF).
Methods:
TRPM7 expression and Ca2+ influx in CF from MI and control mice were quantified by mRNA RT-PCR and whole cell patch clamp technique.
Results:
(1) TRPM7 expression was significantly upregulated post MI and Ca2+ influx of CF were significantly increased post MI [(7.4 +/- 0.7) pA/pF vs. (16.2 +/- 1.7) pA/pF, P < 0.01] and Ca2+ influx of CF increased 3-fold under lower pH condition; (2) These effects could be blocked by knock-out TRPM7 gene with SiRNA.
Conclusion:
TRPM7L upregulation post MI and under lower pH condition are responsible for increased Ca2+ influx in CF.
