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Related Experiment Video

Updated: Jun 27, 2026

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
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A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice

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Modified method for isolation of langerhans islets from mice.

T Kopska1, V Fürstova, J Kovar

  • 1Division of Cell and Molecular Biology & Centre for Research of Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. kopska@centrum.cz

Transplantation Proceedings
|December 23, 2008
PubMed
Summary

This study presents a modified method for isolating Langerhans islets without using Ficoll gradients. This new technique yields comparable or higher numbers of viable, insulin-producing islets, avoiding potential toxicity.

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Area of Science:

  • Endocrinology
  • Islet Biology
  • Transplantation Research

Background:

  • Isolating Langerhans islets is essential for research and potential clinical applications like transplantation.
  • Ficoll gradient centrifugation is a standard method for separating islets from exocrine tissue.
  • Concerns exist regarding Ficoll gradients negatively impacting islet viability and function.

Purpose of the Study:

  • To develop an alternative method for isolating Langerhans islets that omits Ficoll gradient centrifugation.
  • To achieve a yield of viable, functional islets comparable to or better than existing protocols.
  • To mitigate potential toxicity associated with Ficoll gradient use.

Main Methods:

  • Modified islet isolation procedure excluding Ficoll gradient centrifugation.

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Last Updated: Jun 27, 2026

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
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A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
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Published on: August 30, 2019

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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  • Separation based on differential sedimentation rates and attachment properties of islets.
  • Handpicking of purified islets from suspension.

    • Main Results:

    • Achieved a mean yield of 900 viable, insulin-producing islets per mouse.
    • Yield was comparable to or higher than conventional Ficoll-based protocols.
    • The modified method produced highly pure islets.

    Conclusions:

    • Excluding Ficoll gradient centrifugation is a viable modification for Langerhans islet isolation.
    • The new method provides a high yield of functional islets.
    • This approach is beneficial when Ficoll gradient toxicity is a concern.