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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
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Virus analysis using electromigration techniques.

Leopold Kremser1, Dieter Blaas, Ernst Kenndler

  • 1Innsbruck Medical University, Biocenter, Division of Clinical Biochemistry, Innsbruck, Austria. Leopold.Kremser@i-med.ac.at

Electrophoresis
|December 23, 2008
PubMed
Summary

Capillary electrophoresis (CE) and microfluidic devices offer robust analysis of viruses. These advanced techniques enable detection and study of viral interactions and genome release, improving viral diagnostics.

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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Virology

Background:

  • Capillary electrophoresis (CE) and microfluidic devices have advanced virus analysis since 2005.
  • Recent progress focuses on zone electrophoresis and isoelectric focusing (IEF) for viral characterization.

Purpose of the Study:

  • To review advancements in CE and microfluidic analysis of viruses over the past four years.
  • To highlight methodologies for viral detection, interaction studies, and genome analysis.

Main Methods:

  • Zone electrophoresis and isoelectric focusing (IEF) applied to various viruses and bacteriophages.
  • Use of detergents and salts in background electrolyte solutions (BGEs) for robust CE analysis.
  • Analyte detection via UV absorbance and fluorescence labeling of viral components.

Main Results:

  • CE methods successfully analyzed human rhinoviruses, poliovirus, Semliki Forest virus, norovirus-like particles, and bacteriophages MS2 and T5.
  • Detergents and salts are crucial for reproducible CE.
  • Fluorescence detection enhanced sensitivity, enabling monitoring of viral genome release and interactions.

Conclusions:

  • CE and microfluidic devices provide high-resolution analysis of viruses and their interactions.
  • These techniques are valuable for studying viral structure, genome dynamics, and host-pathogen interactions.
  • Further development promises enhanced viral diagnostics and research capabilities.