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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

Amplified in breast cancer 1 expression in breast cancer

M A Thorat1, D Turbin, A Morimiya

  • 1Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Histopathology
|December 23, 2008
PubMed
Abstract

Insights

Amplified in breast cancer 1 (AIB1) expression correlates with HER-2 in breast cancer. AIB1 did not predict survival but supports its role in estrogen receptor-growth factor interactions.

Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Amplified in breast cancer 1 (AIB1) is an estrogen receptor (ER) co-activator.
  • AIB1 may contribute to endocrine resistance by mediating ER-growth factor crosstalk, particularly with HER-2.

Purpose of the Study:

  • To analyze AIB1 expression in breast cancer using immunohistochemistry.
  • To investigate correlations between AIB1 expression and prognostic variables.
  • To determine the effect of AIB1 on patient survival.

Main Methods:

  • Tumor tissue microarrays from 438 breast cancer patients were analyzed.
  • Immunohistochemistry was used to assess AIB1 expression.
  • Correlations with prognostic factors and survival were studied over a median follow-up of 15.4 years.

Main Results:

  • Positive AIB1 expression was observed in 146/395 (37%) breast cancers.
  • AIB1 expression correlated negatively with ER and progesterone receptor (PR) and positively with HER-2 and tumor grade.
  • AIB1 did not correlate with nodal status and did not predict survival in univariate or multivariate analyses.

Conclusions:

  • AIB1 expression is associated with HER-2 expression in breast cancer.
  • A trend towards loss of PR expression was noted in ER-positive tumors with AIB1 expression.
  • The findings support AIB1's role in ER-growth factor interactions.

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