Related Experiment Video
Updated: Jun 26, 2026
10:23
A Computational Modeling Approach to Investigate the Influence of Hyperthermia on the Tumor Microenvironment
Published on: December 1, 2023
Freezing-induced fluid-matrix interaction in poroelastic material
Bumsoo Han1, Jeffrey D Miller, Jun K Jung
1Department of Mechanical and Aerospace Engineering, University of Texas at Arlington, Arlington, TX 76019, USA. bhan@uta.edu
Journal of Biomechanical Engineering
|December 24, 2008
Summary
Freezing biological tissues alters their extracellular matrix (ECM) structure, impacting cryomedicine applications. Understanding these ECM changes is crucial for developing effective tissue-based therapies.
Area of Science:
- Biophysics
- Biomaterials Science
- Tissue Engineering
Background:
- Cryomedicine applications rely on preserving tissue functionality.
- Tissue microstructure, particularly the extracellular matrix (ECM), dictates functionality.
- Precise control over freezing-induced changes in ECM is essential for successful biomedical applications.
Purpose of the Study:
- To investigate the spatiotemporal effects of freezing on the extracellular matrix (ECM) of biological tissues.
- To develop a theoretical model for understanding freezing-induced alterations in ECM microstructure.
- To correlate macroscopic freezing protocols with microstructural changes in tissues.
Main Methods:
- Experimental analysis of type I collagen gel subjected to freezing.
- Theoretical modeling of biological tissue as a poroelastic material.
- Investigation of interstitial fluid transport and its interaction with the ECM during freezing.
Main Results:
- Freezing enlarged the pore structure and increased collagen fibril diameters in the ECM.
- Lower freezing temperatures exacerbated these structural changes.
- Theoretical model indicated fluid transport from the phase change interface, causing matrix pore enlargement.
Conclusions:
- Macroscopic freezing protocols can lead to varied microstructural ECM alterations based on inherent matrix properties.
- These findings are vital for understanding tissue-specific outcomes in cryomedicine.
- The study provides insights for designing optimized cryomedicine applications across diverse tissue types.
Related Concept Videos
01:34Fluid Mosaic Model
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.LipidsThe most...
01:19Fluid Mosaic Model
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich with the analogy of...
02:39Phase Transitions: Melting and Freezing
Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
01:18Newtonian Fluid: Problem Solving
Newtonian fluids exhibit a constant viscosity, meaning their shear stress and shear strain rate are directly proportional. This property ensures a predictable and stable response to applied forces, maintaining a linear relationship between force and flow. Examples include water, air, and light oils, consistently demonstrating this proportional behavior regardless of external conditions.
A velocity gradient forms within the fluid when a Newtonian fluid is placed between two parallel plates, with...
A velocity gradient forms within the fluid when a Newtonian fluid is placed between two parallel plates, with...
01:14Pressure of Fluids
There are many examples of pressure in fluids in everyday life, such as in relation to blood (high or low blood pressure) and in relation to weather (high- and low-pressure weather systems). A given force can have a significantly different effect, depending on the area over which the force is exerted. For instance, a force applied to an area of 1 mm2 has a pressure that is 100 times greater than the same force applied to an area of 1 cm2. That's why a sharp needle is able to poke through skin...
01:17Accelerating Fluids
When a fluid is in constant acceleration, the pressure and buoyant force equations are modified. Suppose a beaker is placed in an elevator accelerating upward with a constant acceleration, a. In the beaker, assume there is a thin cylinder of height h with an infinitesimal cross-sectional area, ΔS.
The motion of the liquid within this infinitesimal cylinder is considered to obtain the pressure difference. Three vertical forces act on this liquid:
The motion of the liquid within this infinitesimal cylinder is considered to obtain the pressure difference. Three vertical forces act on this liquid: