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Updated: Jun 26, 2026
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Published on: October 3, 2014
[Construction of tissue-engineering skin with carrier of active composite dermal matrix]
Zhong-feng Ma1, Jia-ke Chai, Hong-ming Yang
1Burns Institute, the First Affiliated Hospital to the PLA General Hospital, Beijing 100037, PR China.
Objective:
To construct of tissue engineering skin including active composite dermal matrix.
Methods:
The human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.
Results:
The structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.
Conclusion:
Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.
