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Flow cytometric DNA content in Kaposi's sarcoma by histologic stage. Comparison with angiosarcoma
M Dictor1, M Fernö, B Baldetorp
1Department of Pathology, University Hospital, Lund, Sweden.
Analytical and Quantitative Cytology and Histology
|June 1, 1991
Summary
Kaposi
Area of Science:
- Oncology
- Pathology
- Genetics
Background:
- Kaposi's sarcoma (KS) is a multicentric endothelial cell proliferation.
- KS lesions can progress histologically, sometimes resembling angiosarcoma.
- Understanding DNA content in KS progression is crucial for diagnosis.
Purpose of the Study:
- To investigate the relationship between nuclear DNA content and the histological stage of Kaposi's sarcoma.
- To compare the DNA ploidy status of Kaposi's sarcoma with angiosarcomas.
Main Methods:
- Flow cytometric DNA ploidy analysis was performed on 29 paraffin-embedded KS biopsy specimens from 25 cases.
- KS specimens were classified into histological stages (1, 1t2, 2, 2t3, 3).
- DNA ploidy status was compared with 14 angiosarcoma specimens.
Main Results:
- 17 of 25 KS specimens (stage 2 or less) showed diploid DNA distribution.
- 8 KS specimens (stage 2 or less) exhibited peridiploid DNA distribution.
- Advanced stage KS (2t3, 3) and angiosarcomas showed aneuploid or nondiploid DNA distributions.
Conclusions:
- Kaposi's sarcoma exhibits a normal karyotype up to stage 2.
- Atypia in later stages of Kaposi's sarcoma correlates with numerical and structural chromosomal aberrations.
- DNA ploidy analysis can aid in differentiating advanced KS from angiosarcoma.