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Related Experiment Video

Updated: Jun 26, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Published on: October 2, 2018

Caffeic acid derivatives from Eupatorium perfoliatum L.

Mareike Maas¹, Frank Petereit, Andreas Hensel

¹University of Münster, Institute for Pharmaceutical Biology and Phytochemistry, Hittorfstrasse 56, D-48149 Münster, Germany.

Molecules (Basel, Switzerland)

|December 24, 2008

Summary

Researchers identified six caffeic acid derivatives from Eupatorium perfoliatum L. This includes known compounds like chlorogenic acid and three novel glucaric acid depsides, expanding our knowledge of plant-derived compounds.

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Last Updated: Jun 26, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Published on: October 2, 2018

Area of Science:

Phytochemistry
Natural Product Chemistry
Organic Chemistry

Background:

Eupatorium perfoliatum L. (Asteraceae) is a medicinal herb with a history of traditional use.
Caffeic acid derivatives are known for their diverse biological activities.
Understanding the chemical constituents of medicinal plants is crucial for their scientific validation.

Purpose of the Study:

To isolate and identify chemical constituents from Eupatorium perfoliatum L.
To characterize novel caffeic acid derivatives from this plant species.
To contribute to the phytochemical knowledge of Eupatorium perfoliatum.

Main Methods:

Extraction of Eupatorium perfoliatum L. using methanol/water and ethyl acetate.
Isolation of compounds using chromatographic techniques.
Structure elucidation of isolated compounds via 1D- and 2D-NMR spectroscopy.

Main Results:

Six caffeic acid derivatives were isolated and identified.
Three known compounds were identified: 5-caffeoylquinic acid (chlorogenic acid), 3-caffeoylquinic acid (neochlorogenic acid), and 3,5-dicaffeoylquinic acid.
Three previously unknown depsides of caffeic acid with glucaric acid were discovered: 2,5-dicaffeoylglucaric acid, 3,4-dicaffeoylglucaric acid, and 2,4- or 3,5-dicaffeoylglucaric acid.

Conclusions:

The study successfully identified known and novel caffeic acid derivatives from Eupatorium perfoliatum L.
The discovery of new glucaric acid depsides expands the known chemical diversity of this plant.
These findings provide a basis for further investigation into the pharmacological properties of these compounds.