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Updated: Jun 26, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Image contrast enhancement in angular domain optical imaging of turbid media
Fartash Vasefi1, Bozena Kaminska, Glenn H Chapman
1The School of Engineering Science, Simon Fraser University, Burnaby, BC, Canada. fartash@ieee.org
Abstract:
Imaging structures within a turbid medium using Angular Domain Imaging (ADI) employs an angular filter array to separate weakly scattered photons from those that are highly scattered. At high scattering coefficients, ADI contrast declines due to the large fraction of non-uniform background scattered light still within the acceptance angle. This paper demonstrates various methods to enhance the image contrast in ADI. Experiments where a wedge prism was used to deviate the laser source so that scattered photons could be imaged and subtracted from the image obtained by standard ADI provided the greatest improvement in image contrast.
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