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Updated: Aug 11, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
[Circulating lymphocyte subpopulations in patients with Crohn's disease]
Activated lymphocytes may indicate Crohn's disease activity. While T cell subsets remain stable, elevated activated lymphocytes (HLA-DR, CD25) signal disease exacerbation, suggesting a potential biomarker for monitoring Crohn's disease progression.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Context:
- Crohn's disease is a chronic inflammatory bowel disease.
- Assessing disease activity is crucial for effective patient management.
- Current biomarkers for Crohn's disease activity have limitations.
Purpose:
- To investigate the role of lymphocyte subsets and activation markers in Crohn's disease.
- To determine if lymphocyte phenotypes correlate with disease activity in Crohn's disease patients.
Summary:
- This study enumerated circulating lymphocytes in Crohn's disease patients and healthy controls using immunofluorescence and monoclonal antibodies.
- No significant differences were found in T cell proportions (CD 3, CD 4, CD 8) or B cell markers between patients and controls.
- However, increased numbers of activated lymphocytes expressing HLA-DR and CD25 were observed during acute exacerbations of Crohn's disease.
- In remission, the population of activated lymphocytes was within the upper normal range.
Impact:
- The findings suggest that activated lymphocyte assessment may serve as a valuable parameter for evaluating Crohn's disease activity.
- This could lead to improved monitoring and personalized treatment strategies for Crohn's disease.
- Further research can validate these findings and integrate them into clinical practice for better patient outcomes.
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