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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: Jun 26, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Mass spectrometry based targeted protein quantification: methods and applications.

Sheng Pan1, Ruedi Aebersold, Ru Chen

  • 1Department of Pathology, University of Washington, Seattle, Washington 98195, USA. span@u.washington.edu

Journal of Proteome Research
|December 25, 2008
PubMed
Summary
This summary is machine-generated.

New mass spectrometry technologies enable broad, precise protein quantification for clinical research. This approach uses stable isotope labeled standards for accurate biomarker discovery and development.

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Last Updated: Jun 26, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

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Published on: August 17, 2015

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11:49

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10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Proteomics
  • Clinical Research
  • Biomarker Development

Background:

  • Mass spectrometry-based targeted protein quantification has advanced significantly.
  • These advancements enable a wider range of proteomic applications in clinical settings.

Purpose of the Study:

  • To review recently developed platform technologies for clinical proteomics.
  • To highlight emerging applications in biomarker development.

Main Methods:

  • Utilizing a "universal" approach for quantitative assays across a wide spectrum of proteins.
  • Employing synthetic stable isotope labeled peptides or proteins as internal standards.
  • Assembling multiplex detections within a single measurement.

Main Results:

  • Demonstrated capability for broad protein quantification with minimal restrictions.
  • Facilitated ease of multiplexed assays in single measurements.
  • Enabled precise quantification of candidate proteins using internal standards.

Conclusions:

  • Recent technological progress in mass spectrometry enhances clinical proteomics.
  • Developed platform technologies support emerging applications and biomarker discovery.