Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Foxp3 drives context-dependent epigenetic programs that define regulatory T cell molecular identity and function.

Science immunology·2026
Same author

PD-1 antibody-bound progenitor-exhausted CD8<sup>+</sup> T cells in lymph nodes boost PD-1-blockade anti-tumor immunity in gastrointestinal cancer.

Nature communications·2026
Same author

Stable and functional regulatory T cell attenuates fibrotic remodeling in heart failure.

Regenerative therapy·2026
Same author

T Cell Receptor Signaling and Immune Tolerance: From Autoimmunity to Cancer Immunity.

Annual review of immunology·2026
Same author

ROSE12, a novel anti-CTLA-4 FcγRs binding-enhanced antibody activated by extracellular adenosine triphosphate, shows tumor-selective regulatory T-cell depletion and antitumor efficacy without systemic immune activation.

Journal for immunotherapy of cancer·2026
Same author

Prostaglandin E<sub>2</sub>-EP2/EP4 signaling induces the tumor-infiltrating Treg phenotype for tumor growth.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 26, 2026

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
12:30

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

A simple detection method for low-affinity membrane protein interactions by baculoviral display.

Toshiko Sakihama1, Takato Sato, Hiroko Iwanari

  • 1Department of Molecular Biology and Medicine, The University of Tokyo, Tokyo, Japan. sakihama@med.rcast.u-tokyo.ac.jp

Plos One
|December 25, 2008
PubMed
Summary

This study demonstrates a novel baculovirus display system for detecting membrane protein interactions crucial for cell communication. This method effectively identifies interactions between immune system proteins like CD2 and CD58, offering a powerful tool for biological research.

More Related Videos

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Related Experiment Videos

Last Updated: Jun 26, 2026

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
12:30

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Area of Science:

  • Biochemistry
  • Immunology
  • Molecular Biology

Background:

  • Membrane protein interactions are vital for cell recognition in the immune and neural systems.
  • A significant challenge in studying membrane proteins is expressing them in an active, functional form.
  • Baculovirus particles (budded virus, BV) have emerged as a platform for displaying functional membrane proteins.

Purpose of the Study:

  • To apply the baculovirus display system for detecting interactions between immune system membrane proteins.
  • To validate the efficacy of the BV display system in identifying receptor-ligand interactions.

Main Methods:

  • Infection of Sf9 cells with recombinant baculovirus encoding T cell membrane proteins (CD2, CD58, CD40, CD40L, GITR, GITRL).
  • Detection of protein interactions using enzyme-linked immunosorbent assay (ELISA) and flow cytometry.
  • Isolation of cDNA using magnetic separation with BV-displayed proteins.

Main Results:

  • Specific interactions were detected between CD2-displaying BV and CD58-displaying BV.
  • Interactions between CD40-CD40L and GITR-GITRL pairs were also confirmed using the BV display system.
  • BV displaying ligands showed specific binding to cells expressing their corresponding receptors, and cDNA was successfully isolated.

Conclusions:

  • The baculovirus display system is effective for detecting membrane protein interactions.
  • This system can display various membrane proteins and their complexes in their native form.
  • The BV display system holds significant potential for discovering natural ligands and developing screening platforms for therapeutics.