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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 26, 2026

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

Microarray of DNA-protein complexes on poly-3-hydroxybutyrate surface for pathogen detection.

Tae Jung Park¹, Seung Min Yoo, Ki Chang Keum

¹Department of Chemical and Biomolecular Engineering (BK21 program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea.

Analytical and Bioanalytical Chemistry

|December 25, 2008

Summary

A new method immobilizes DNA probes on poly-3-hydroxybutyrate (PHB) surfaces using a protein domain. This DNA-protein microarray successfully detects common bacterial pathogens, offering a versatile diagnostic tool.

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Last Updated: Jun 26, 2026

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

Biomaterials Science
Molecular Diagnostics
Biotechnology

Background:

Specific immobilization of DNA probes is crucial for diagnostic microarrays.
Poly-3-hydroxybutyrate (PHB) offers a biocompatible surface for biomolecule attachment.
Existing methods often require chemical modification of biomolecules or surfaces.

Purpose of the Study:

To develop a novel strategy for specific DNA probe immobilization on PHB surfaces.
To create a DNA-protein complex microarray for clinical pathogen detection.
To demonstrate the platform's utility without chemical modification.

Main Methods:

Utilized the substrate-binding domain (SBD) of PHB depolymerase for specific binding.
Fused core streptavidin (cSA) to SBD for biotin-labeled DNA probe immobilization.
Designed pathogen-specific oligonucleotide probes and used PCR for target DNA preparation.
Microspotted cSA-SBD-probe complexes onto PHB-coated slides.

Main Results:

Successfully developed a DNA-protein complex microarray on PHB.
The microarray accurately diagnosed four model pathogens: Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa.
Pathogen detection was successful even in the presence of other microorganisms.

Conclusions:

The developed DNA-protein complex microarray platform is effective for pathogen detection.
This PHB and SBD-based technology enables DNA-DNA and DNA-biomolecule interaction detection.
The method avoids synthetic or chemical modifications, simplifying biomolecule immobilization.