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Related Experiment Video

Updated: Jun 26, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

PTPN22 C1858T polymorphism and human brucellosis.

Maria Jose Bravo¹, Juan Dios Colmenero, Maria Isabel Queipo-Ortuño

¹Immunology Service, Carlos Haya Regional University Hospital, Avda. Carlos Haya 82, 29010 Malaga, Spain. mariajose.bravo@fundacionimabis.org

Scandinavian Journal of Infectious Diseases

|December 25, 2008

Summary

The PTPN22 gene variant, previously linked to autoimmune diseases, was investigated for its role in human brucellosis susceptibility. This study found no significant association between the PTPN22 1858CT variant and brucellosis.

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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

Area of Science:

Immunogenetics
Infectious Diseases

Background:

The PTPN22 gene encodes lymphoid-specific phosphatase (Lyp), a negative regulator of T-cell activation.
A single nucleotide polymorphism (SNP) in PTPN22 (1858CT) is associated with autoimmune disorders and tuberculosis susceptibility.

Purpose of the Study:

To investigate the potential association between the PTPN22 1858CT variant and susceptibility to human brucellosis, caused by Brucella melitensis.

Main Methods:

Genotyping of the PTPN22 1858CT polymorphism was performed using an allele discrimination assay with TaqMan 5'.
A case-control study was conducted involving 111 brucellosis patients and 150 healthy controls.

Main Results:

No statistically significant differences were observed in the genotype or allele frequencies of PTPN22 1858CT between brucellosis patients and healthy controls.
The PTPN22 1858CT variant does not appear to be a risk factor for developing human brucellosis.

Conclusions:

The PTPN22 1858CT variant is not associated with susceptibility to human brucellosis.
Further research may be needed to identify genetic factors influencing brucellosis risk.