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Updated: Jun 26, 2026
07:13
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
Published on: April 26, 2011
Clinical use of circulating nucleosomes
Stefan Holdenrieder1, Petra Stieber
1Institute of Clinical Chemistry, University Hospital, Munich, Germany. Stefan.Holdenrieder@med.uni-muenchen.de
Critical Reviews in Clinical Laboratory Sciences
|December 25, 2008
Summary
Elevated circulating nucleosomes, DNA-protein complexes, signal various diseases including cancer and sepsis. Monitoring these nucleosomes aids in early diagnosis and predicting treatment response.
Area of Science:
- Biochemistry
- Cell Biology
- Clinical Diagnostics
Background:
- Nucleosomes are released from stressed or dying cells into circulation.
- Elevated circulating nucleosome levels are observed in cancers, sepsis, stroke, trauma, and autoimmune diseases.
Purpose of the Study:
- To review the structural and functional properties of nucleosomes.
- To explore the sources, metabolism, and pathophysiological roles of circulating nucleosomes.
- To assess the diagnostic and prognostic relevance of circulating nucleosomes in various diseases.
Main Methods:
- Literature review focusing on nucleosome biology and clinical applications.
- Analysis of studies investigating circulating nucleosomes in disease states.
- Evaluation of nucleosomes as diagnostic and prognostic biomarkers.
Main Results:
- Nucleosomes possess significant pathophysiological roles.
- Circulating nucleosomes are relevant biomarkers for non-malignant conditions like sepsis and autoimmune diseases.
- Nucleosomes show potential for cancer diagnosis, staging, prognosis, and monitoring therapy response, especially early prediction of response to cytotoxic therapy.
Conclusions:
- Circulating nucleosomes are valuable indicators in various pathological conditions.
- Nucleosomes represent a promising new diagnostic tool for early cancer therapy response estimation.
- Further research into nucleosome metabolism and function can enhance their clinical utility.
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