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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Related Experiment Video

Updated: Jun 26, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Advances in enantioselective separations using electromigration capillary techniques.

Beatrix Preinerstorfer1, Michael Lämmerhofer, Wolfgang Lindner

  • 1Department of Analytical Chemistry and Food Chemistry, Christian Doppler Laboratory for Molecular Recognition Materials, University of Vienna, Vienna, Austria.

Electrophoresis
|December 25, 2008
PubMed
Summary

This review covers recent advances in chiral separations using electromigration techniques. It highlights new chiral selectors and separation mechanisms for analyzing pharmaceuticals and other chiral compounds.

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Last Updated: Jun 26, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

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Published on: April 23, 2019

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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Published on: December 22, 2017

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Chiral compounds are crucial in pharmaceuticals, agrochemicals, and biochemistry.
  • Enantioselective separations and stereoselective analyses are vital for quality control and research.
  • Electromigration techniques offer powerful methods for chiral analysis.

Purpose of the Study:

  • To review recent literature (2007-mid 2008) on enantioselective separations and stereoselective analyses of chiral entities.
  • To focus on advancements in electromigration techniques like capillary electrophoresis (CE) and microchip CE.
  • To detail new chiral selector systems, separation mechanisms, and applications.

Main Methods:

  • Comprehensive literature review of studies published between 2007 and mid-2008.
  • Analysis of research utilizing capillary electrophoresis (CE), micellar electrokinetic chromatography (MEKC), microchip electrophoresis (MEEKC), capillary electrochromatography (CEC), and microchip CE.
  • Focus on studies describing novel chiral selectors and separation mechanisms.

Main Results:

  • Identification of new chiral selector systems for improved enantioselective separations.
  • Elucidation of separation mechanisms underlying stereoselective analyses in various electromigration techniques.
  • Demonstration of applications in the analysis of pharmaceuticals, phytochemicals, biochemicals, agrochemicals, and fine chemicals.

Conclusions:

  • Electromigration techniques continue to evolve for effective chiral analysis.
  • Advancements in chiral selectors and understanding separation mechanisms enhance analytical capabilities.
  • The reviewed literature showcases significant progress in the stereoselective analysis of diverse chiral compounds.