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Rapid preparation of lysate for immunoprecipitation using a low protein-binding micro-filter
M Ayres1, H B Marshall, J Ishikawa
1Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.
Biotechniques
|May 1, 1991
Abstract:
A simple method to prepare cell lysate for immunoprecipitation is described. The procedure utilizes filtration of cell lysates by using a low protein-binding filter. This filtration method gave an equivalent result to that of the centrifugation method.