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Separation of carbohydrates and carbohydrate derivatives by HPLC with cation-exchange columns at high pH
D W Togami1, B J Poulsen, C W Batalao
1Interaction Chemicals Inc., Mountain View, CA 94043.
Biotechniques
|May 1, 1991
Abstract:
Cation-exchange columns were found to be stable when used at high pH and high temperature for high-performance liquid chromatographic separations of carbohydrates and carbohydrate derivatives. Pulsed amperometric detection and refractive index detection were found to be suitable detection modes with these cation-exchange columns. Significant differences in carbohydrate separation selectivity were observed between cation-exchange and anion-exchange columns.