Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
Adrenergic Agonists: Indirect-Acting Agents01:25

Adrenergic Agonists: Indirect-Acting Agents

Indirect-acting adrenergic agonists potentiate the effects of endogenous catecholamines through different mechanisms without directly binding to adrenoceptors.
One mechanism involves depleting stored catecholamines by displacing them from synaptic vesicles. These agents, known as "displacers," are transported into vesicles at the expense of noradrenaline. Examples include amphetamine and tyramine, which lack a catechol moiety, resulting in prolonged action, improved oral bioavailability, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrent fatal hemolytic anemia associated with gross liver damage and splenomegaly.

The American journal of the medical sciences·2010
Same author

Brain amine concentrations after monoamine oxidase inhibitor administration.

British medical journal·1972
Same author

5-hydroxytryptamine, noradrenaline, and dopamine in brainstem, hypothalamus, and caudate nucleus of controls and of patients committing suicide by coal-gas poisoning.

Lancet (London, England)·1969
Same author

Action of adenosine diphosphate on human platelets.

Experientia·1968
Same author

Distribution and localization of indolealkylamines. Reporter's remarks.

Advances in pharmacology·1968
Same author

Platelet function tests in thrombocythaemia and thrombocytosis.

British journal of haematology·1966

Related Experiment Video

Updated: Jun 26, 2026

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Uptake of 5-hydroxytryptamine by platelets.

R S Stacey

    British Journal of Pharmacology and Chemotherapy
    |December 25, 2008
    PubMed
    Summary

    Human blood platelets exhibit distinct serotonin (5-hydroxytryptamine) and tryptamine uptake mechanisms. Tryptamine uptake is less specific, while other substances like imipramine can inhibit serotonin uptake by platelets.

    Area of Science:

    • Pharmacology
    • Biochemistry
    • Cell Biology

    Background:

    • Platelets play a crucial role in hemostasis and serotonin transport.
    • Understanding amine uptake mechanisms in platelets is vital for various physiological and pathological processes.

    Purpose of the Study:

    • To investigate and compare the uptake kinetics of 5-hydroxytryptamine (serotonin) and tryptamine in human blood platelets.
    • To identify factors influencing amine uptake and potential inhibitors.

    Main Methods:

    • Incubation of human blood platelets with 5-hydroxytryptamine and tryptamine.
    • Measurement of amine uptake under varying conditions (concentration, temperature, pH).
    • Assessment of competitive inhibition and effects of other substances on uptake.

    More Related Videos

    Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
    08:04

    Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

    Published on: June 10, 2025

    Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
    09:13

    Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

    Published on: April 6, 2017

    Related Experiment Videos

    Last Updated: Jun 26, 2026

    A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
    07:56

    A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

    Published on: August 11, 2021

    Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
    08:04

    Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

    Published on: June 10, 2025

    Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
    09:13

    Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

    Published on: April 6, 2017

    Main Results:

    • Tryptamine uptake showed linear dependence on concentration, temperature independence, and direct pH correlation.
    • Co-incubation with tryptamine competitively inhibited 5-hydroxytryptamine uptake.
    • Imipramine, cocaine, and chlorpromazine were potent inhibitors of 5-hydroxytryptamine uptake.

    Conclusions:

    • Human platelets possess distinct uptake systems for 5-hydroxytryptamine and tryptamine.
    • Tryptamine's interaction with the 5-hydroxytryptamine uptake system is competitive.
    • Platelet amine uptake is modulated by various pharmacological agents, suggesting potential therapeutic targets.