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Updated: Jun 26, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

High-resolution surface-plasmon resonance real-time imaging.

R Vander¹, S G Lipson

¹Department of Physics, Technion-Israel Institute of Technology, Haifa, Israel.

|December 26, 2008

Summary

This summary is machine-generated.

This study demonstrates speckle-free, high-resolution imaging of transparent dielectric samples using surface-plasmon resonance and radially polarized light. This technique offers high sensitivity for detecting refractive index changes in real-time.

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Last Updated: Jun 26, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

Area of Science:

Optics and Photonics
Materials Science
Surface Science

Background:

Surface-plasmon resonance (SPR) is a powerful technique for sensing at interfaces.
High-resolution imaging of transparent dielectric materials presents challenges.
Real-time monitoring of refractive index changes is crucial for various applications.

Purpose of the Study:

To develop a novel method for high-resolution, real-time imaging of transparent dielectric samples.
To utilize radially polarized illumination for improved image quality in SPR.
To quantify the sensitivity and spatial resolution of the developed imaging technique.

Main Methods:

Employing surface-plasmon resonance (SPR) with a silver film.
Utilizing radially polarized illumination from a 530 nm LED.
Achieving speckle-free imaging with high spatial resolution.
Measuring the modulation transfer function (MTF).

Main Results:

High-resolution, real-time images of transparent dielectric samples were obtained.
Speckle-free images with uniform high spatial resolution were achieved using radial polarization.
Sensitivity to refractive index changes was demonstrated to be better than 10^-3.
The modulation transfer function was measured up to a spatial frequency of 1 µm^-1.

Conclusions:

Radially polarized illumination significantly enhances SPR imaging quality for transparent dielectrics.
The developed SPR imaging method offers high sensitivity and resolution for real-time analysis.
This technique provides a valuable tool for studying transparent materials at interfaces.