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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
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Published on: February 27, 2012

High-throughput RNAi screen in Drosophila.

Ramanuj DasGupta1, Foster C Gonsalves

  • 1Department of Pharmacology, New York University School of Medicine/Cancer Institute, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 26, 2008
PubMed
Summary

High-throughput screening (HTS) and RNA-interference (RNAi) enable systematic discovery of gene functions. This powerful technology aids in identifying novel modulators of cell-signaling pathways for genome-scale screens.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Functional Genomics

Background:

  • Genetic analyses have identified key regulatory genes, but many gene functions remain unknown.
  • Large-scale genome projects present challenges in deriving biological information from raw sequence data.

Purpose of the Study:

  • To discuss the application, advantages, and limitations of RNA-interference (RNAi) technology.
  • To explore the utility of RNAi in identifying novel modulators of cell-signaling pathways.
  • To assess the future scope of RNAi in genome-scale screening.

Main Methods:

  • High-throughput screening (HTS) technologies for gene-function discovery.
  • RNA-interference (RNAi) using double-stranded RNA (dsRNA) to suppress gene expression.
  • Cell-based assays to query gene function in biological processes.

Main Results:

  • RNAi is a powerful tool for unbiased, systematic, and time-efficient gene-function discovery.
  • RNAi facilitates the identification of novel modulators in cell-signaling pathways.
  • RNAi enables functional interrogation of entire genomes using cell-based assays.

Conclusions:

  • RNAi technology is crucial for discovering the function of unannotated genes.
  • RNAi significantly advances functional genomics and the study of cell signaling.
  • RNAi holds great potential for designing more efficient genome-scale screens.