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Published on: March 14, 2011
Oxidative stress and delta-ALA-D activity in different conditioning regimens in allogeneic bone marrow
Thissiane L Gonçalves1, Dalila M Benvegnú, Gabriela Bonfanti
1Departamento de Química, CCNE, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. thissianegoncalves@yahoo.com.br
Objectives:
To compare different conditioning regimens (CR), in order to determine whether either of them could be less toxic to allogeneic bone marrow transplantation (BMT) patients in terms of oxidative stress and also analyze delta-ALA-D activity as a possible marker of oxidative stress.
Design And Methods:
Lipid peroxidation, vitamin C, thiol groups levels and catalase, superoxide dismutase and delta-ALA-D activity were determined in 21 healthy controls, 5 patients with fludarabine+cyclophosphamide (FluCy) CR, 12 with busulfan+cyclophosphamide (BuCy) and 4 with cyclophosphamide+total body irradiation (CyTBI).
Results:
There were a decrease in enzymatic and non enzymatic antioxidants, in delta-ALA-D activity, and in all CRs and an increase in lipid peroxidation more pronounced in CyTBI CR.
Conclusions:
All CRs promoted oxidative stress in allogeneic BMT patients, but this was more pronounced with CyTBI and delta-ALA-D activity seemed to be an additional biomarker of oxidative stress in these patients.
