Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: World mental health day: mental health in the workplace.

Frontiers in public health·2026
Same author

Geospatial insights and artificial intelligence in West Nile virus disease Epidemiology: The Italy case study.

Public health·2026
Same author

Serum peripherin as a disease biomarker in hereditary transthyretin amyloidosis: a multicenter cohort study.

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis·2026
Same author

Editorial: Research advances and challenges in emerging and re-emerging viral diseases.

Frontiers in microbiology·2026
Same author

Seroprevalence of Influenza D Virus in Cattle Workers: An Occupational Health Perspective.

Journal of medical virology·2026
Same author

Heat stress and neurological biomarkers in outdoor workers: a cross-sectional observational study.

Environmental research·2025

Related Experiment Video

Updated: Jun 26, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Proteome-based epitopic peptide scanning along PSA.

Angela Stufano1, Darja Kanduc

  • 1Department of Biochemistry and Molecular Biology, University of Bari, Via Orabona 4, Bari 70125, Italy.

Experimental and Molecular Pathology
|December 27, 2008
PubMed
Summary

Defining the immunogenic peptide repertoire from tumor-associated antigens (TAAs) is key for immunotherapy. Research identified a specific PSA peptide motif crucial for anti-PSA antibody binding, aiding in cancer treatment design.

More Related Videos

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Related Experiment Videos

Last Updated: Jun 26, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Area of Science:

  • Immunology
  • Oncology
  • Biochemistry

Background:

  • Effective cancer immunotherapy relies on understanding the immunogenic peptide repertoire derived from tumor-associated antigens (TAAs).
  • Generating immunogenic peptides involves multiple complex mechanisms.
  • Prostate-specific antigen (PSA) is a key TAA in prostate cancer.

Purpose of the Study:

  • To investigate the factors influencing the immunogenicity of prostate-specific antigen (PSA) peptides.
  • To identify the specific peptide motif of PSA responsible for binding to a murine monoclonal antibody (MAb).
  • To analyze the role of peptide similarity to the murine proteome, affinity to H2-Ad/Ed molecules, and proteasomal cleavage in immunogenicity.

Main Methods:

  • Utilized a murine monoclonal antibody (MAb) ER-PR8 raised against human PSA as an experimental model.
  • Investigated PSA peptide characteristics including sequence similarity to the murine proteome, H2-Ad/Ed binding affinity, and proteasomal cleavage patterns.
  • Identified the core interaction motif between the anti-PSA MAb and PSA.

Main Results:

  • The core interaction between the anti-PSA MAb ER-PR8 and PSA resides in the pentapeptide motif PSA(27-31)GGWEC.
  • This critical pentapeptide motif exhibits no significant sequence similarity to the murine proteome.
  • The identified motif also possesses a low number of potential proteolytic cleavage sites.

Conclusions:

  • The PSA(27-31)GGWEC pentapeptide is a key determinant of the interaction with the anti-PSA MAb ER-PR8.
  • Lack of murine proteome similarity and limited proteolytic sites contribute to the immunogenic potential of this PSA peptide.
  • These findings are crucial for designing targeted immunotherapies for prostate cancer.