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Related Experiment Video

Updated: Jun 26, 2026

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

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Matrigel cytometry: a novel method for quantifying angiogenesis in vivo.

A Adini¹, O Fainaru, T Udagawa

¹Vascular Biology Program, Department of Surgery, Children's Hospital, and the Department of Ophthalmology, both at Harvard Medical School, Boston, Massachusetts, USA. avner.adini@childrens.harvard.edu

Journal of Immunological Methods

|December 27, 2008

Summary

This study introduces a modified Matrigel plug assay for quantifying in vivo angiogenesis. The enhanced method accurately measures endothelial cell migration and proliferation, offering a better assessment of blood vessel formation rates.

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Area of Science:

Biomedical Engineering
Cell Biology
Vascular Biology

Background:

In vivo angiogenesis evaluation methods often lack precise quantification.
The Matrigel plug assay is a common in vivo model for studying angiogenesis.
Existing methods struggle to accurately measure endothelial cell incorporation into Matrigel plugs.

Purpose of the Study:

To present a modified Matrigel plug assay with improved quantification of endothelial cell incorporation.
To develop a more accurate method for assessing in vivo angiogenesis.
To enable the determination of endothelial cell migration and proliferation rates.

Main Methods:

Modification of the traditional Matrigel plug assay.
Subcutaneous injection of Matrigel plugs containing angiogenic factors into mice.
Protease treatment of retrieved Matrigel plugs to isolate intact cells.
Staining isolated cells with endothelial cell-specific markers.
Quantification of stained cells using flow cytometry (FACS analysis).

Main Results:

The modified assay successfully quantifies endothelial cell incorporation into Matrigel plugs.
Flow cytometry analysis provides precise cell counts.
The method allows for the determination of migrating and proliferating endothelial cells.
This reflects a more accurate measurement of the angiogenesis rate.

Conclusions:

The combination of the Matrigel assay with FACS analysis significantly improves the quantification of in vivo angiogenesis.
This novel approach provides a reliable method to determine the rate of angiogenesis by measuring endothelial cell dynamics.
The enhanced assay offers a valuable tool for research in vascular biology and regenerative medicine.