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Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent – the...

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Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

Towards a 'universal' nucleant for protein crystallization.

Emmanuel Saridakis1, Naomi E Chayen

  • 1Laboratory of Structural and Supramolecular Chemistry, Institute of Physical Chemistry, 15310 Athens, Greece.

Trends in Biotechnology
|December 27, 2008
PubMed
Summary
This summary is machine-generated.

Researchers are seeking a universal nucleant to improve protein crystallization for drug design. Advances in engineered and natural surfaces show promise for controlling nucleation, a critical step in crystal formation.

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Last Updated: Jun 26, 2026

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
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Published on: March 13, 2021

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Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
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Published on: August 14, 2018

Area of Science:

  • Crystallography
  • Biotechnology
  • Materials Science

Background:

  • Protein structure determination is crucial for rational drug design and biotechnology.
  • High-quality protein crystal formation is a significant bottleneck in research progress.
  • Controlling crystal nucleation, the initial step, is key to overcoming crystallization challenges.

Purpose of the Study:

  • To investigate advances in the search for a universal nucleant for protein crystallization.
  • To highlight the efficacy of engineered and naturally structured surfaces in inducing protein nucleation.

Main Methods:

  • Utilizing X-ray diffraction in crystallography to determine atomic structures.
  • Exploring the use of specifically engineered surfaces as nucleants.
  • Investigating naturally structured surfaces for their nucleation capabilities.

Main Results:

  • Demonstrated success with engineered surfaces in promoting protein nucleation.
  • Showcased the effectiveness of naturally structured surfaces in initiating crystallization.
  • Progress reported in the ongoing search for a universal nucleant.

Conclusions:

  • Engineered and natural surfaces represent significant advances in controlling protein nucleation.
  • These surfaces offer potential solutions to the challenge of obtaining high-quality protein crystals.
  • Further development in nucleant technology could accelerate drug design and biotechnology applications.