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Updated: Jun 26, 2026

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
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The Results of Mancini's test depend on the presence bound metal cations in the test protein

S B Cheknyov1, E A Denisova, E M Mongush

  • 1Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. cheknev@riem.ru

Bulletin of Experimental Biology and Medicine
|December 27, 2008
PubMed

Samples of human serum gamma-globulin with specifically bound copper or zinc cations were studied in Mancini's radial immunodiffusion test with human antibodies to IgG (H+L). The intensity of antibody binding to zinc-modified protein was 10-20% higher in comparison with the reference sample, while detection of gamma-globulin with bound copper by antibodies was 20-30% lower than in the corresponding reference sample. Comparison with the results of native gamma-globulin testing indicates limitations of Mancini's method as the quantitative assay for practical diagnosis, because under certain clinical conditions the traditional method can give over- and underestimated results.

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