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Related Experiment Video

Updated: Jun 26, 2026

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

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Tissue procurement for molecular studies using laser-assisted microdissection.

Ulrich Lehmann1, Kreipe Hans

  • 1Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|December 27, 2008
PubMed
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Properly collected human specimens enable molecular disease study. This chapter details best practices for collecting, storing, and processing bone marrow trephines for pure cell isolation using laser-assisted microdissection.

Area of Science:

  • Biomedical research
  • Molecular pathology
  • Tissue processing

Background:

  • Human specimens are crucial for in vivo molecular disease research.
  • Standardized protocols are needed for reliable sample collection and storage.
  • Precise isolation of specific cell populations is essential for accurate molecular analysis.

Purpose of the Study:

  • To highlight critical considerations before collecting and storing human tissues for molecular studies.
  • To provide detailed protocols for bone marrow trephine fixation and processing.
  • To describe the isolation of pure cell populations from bone marrow using laser-assisted microdissection.

Main Methods:

  • Development of protocols for human tissue sample collection and storage.
  • Detailed fixation and processing techniques for bone marrow trephines.

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Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Related Experiment Videos

Last Updated: Jun 26, 2026

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
09:09

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

Laser Microdissection for Species-Agnostic Single-Tissue Applications
08:57

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

  • Application of laser-assisted microdissection for pure cell isolation.

    • Main Results:

    • Established guidelines for human specimen collection and storage for molecular research.
    • Validated protocols for bone marrow trephine fixation and processing.
    • Successful isolation of morphologically and immunohistochemically defined pure cell populations.

    Conclusions:

    • Properly collected and stored human specimens are invaluable for molecular disease research.
    • Standardized protocols ensure the integrity of tissue samples for downstream analysis.
    • Laser-assisted microdissection enables precise isolation of specific cell types from complex tissues.