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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

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Related Experiment Video

Updated: Jun 26, 2026

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells
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Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Published on: May 12, 2023

Tracking gene-modified T cells in vivo.

Alessandra Recchia1, Fulvio Mavilio

  • 1Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|December 27, 2008
PubMed
Summary

Monitoring genetically modified cells in peripheral blood is crucial for patients undergoing gene therapy. This analysis, particularly of T lymphocytes, tracks gene transfer efficiency and potential side effects using FACS and PCR techniques.

Area of Science:

  • Biotechnology
  • Immunology
  • Hematology

Background:

  • Clinical follow-up of gene therapy patients requires monitoring of genetically modified cells.
  • Peripheral blood analysis offers insights into gene transfer efficiency and cell characteristics.
  • Early detection of adverse effects is vital in gene therapy.

Purpose of the Study:

  • To describe techniques for identifying and analyzing genetically modified cells in peripheral blood.
  • To provide guidance on the rational clinical application of these monitoring methods.
  • To highlight the importance of tracking gene-marked T lymphocytes.

Main Methods:

  • Flow cytometry (FACS)-based techniques for cell identification and quantification.
  • Polymerase chain reaction (PCR)-based methods for detecting gene modifications.

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Last Updated: Jun 26, 2026

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  • Analysis of T lymphocytes due to their abundance and longevity.

    • Main Results:

    • FACS and PCR are effective for tracking gene-marked T cells.
    • These methods allow monitoring of both directly modified T cells and those from modified hematopoietic stem cells.
    • The techniques provide crucial data for assessing gene transfer and patient safety.

    Conclusions:

    • Monitoring genetically modified cells, especially T lymphocytes, is essential for hematopoietic cell gene therapy.
    • FACS and PCR offer robust methods for this analysis in a clinical setting.
    • These techniques aid in evaluating treatment efficacy and patient safety.