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Updated: Jun 26, 2026

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

Proteomics studies after hematopoietic stem cell transplantation.

Eva M Weissinger¹, Petra Zürbig, Arnold Ganser

¹Department of Hematology, Hemostasis, and Oncology, Hannover Medical School, Hannover, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|December 27, 2008

Summary

This summary is machine-generated.

Researchers analyzed human urine using capillary electrophoresis and mass spectrometry to find biomarkers for complications after stem cell transplants. This proteomic approach helps identify new therapeutic targets for patient health.

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Intrafemoral Injection of Human Hematopoietic Stem and Progenitor Cells into Immunocompromised Mice

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Published on: December 8, 2023

Area of Science:

Biochemistry
Proteomics
Biomarker Discovery

Background:

Complex biological samples, like body fluids, contain vital health and disease information.
The human proteome, with over 1,000,000 functional protein entities, offers richer insights than the genome.
Analyzing protein and peptide expression in body fluids is crucial for understanding health status.

Purpose of the Study:

To apply capillary electrophoresis (CE) coupled online to electrospray-ionization time-of-flight mass spectrometry (ESI-TOF-MS).
To analyze human urine for identifying specific biomarkers related to complications after allogeneic hematopoietic stem cell transplantation (HSCT).
To enable the sequencing of native proteins/peptides for discovering novel therapeutic targets.

Main Methods:

Online coupling of capillary electrophoresis (CE) with electrospray-ionization time-of-flight mass spectrometry (ESI-TOF-MS).
Analysis of human urine samples.
Proteomic analysis focusing on protein and peptide expression.

Main Results:

Identification of potential biomarkers in human urine specific to HSCT complications.
Demonstration of CE-ESI-TOF-MS as an effective tool for urinary proteomic analysis.
Facilitation of native protein/peptide sequencing.

Conclusions:

Proteomic analysis of urine using CE-ESI-TOF-MS is a powerful method for identifying HSCT complication biomarkers.
This technique aids in the discovery of new therapeutic targets.
Understanding proteomic changes in body fluids is key to advancing personalized medicine and patient care.