Transcriptome targets of the exosome complex in plants

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mRNA Interactome Capture from Plant Protoplasts

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Dmitry Belostotsky¹

¹Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri, Kansas City, Missouri, USA.

Methods in Enzymology

|December 30, 2008

Abstract:

The exosome complex is endowed with the capabilities to conduct 3'-end RNA processing, 3'-end degradation, and surveillance of various RNA substrates. Although the exosome is present in both eukaryotes and archaea, where it plays a central role in cellular RNA metabolism, a substantial degree of variability exists in its modus operandi in the different domains of life. This chapter discusses features of the exosome in plants that distinguish it from the exosome in archaea, fungi, and animals, as well as reviews the resources and tools that are needed to identify and catalog plant exosome targets on a transcriptome-wide scale.

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