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Related Experiment Video

Updated: Jun 26, 2026

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

YAP and p73: a complex affair.

Julian Downward¹, Subham Basu

¹Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. julian.downward@cancer.org.uk

|December 30, 2008

Summary

The Yes-associated protein (YAP) interacts with p73 to initiate PML transcription. This interaction triggers a feedback loop, enhancing p73 activity and stabilizing YAP, particularly during DNA damage responses.

Area of Science:

Cellular biology
Molecular oncology
Gene regulation

Background:

The Hippo-YAP pathway regulates organ size and tumor suppression.
p73 is a tumor suppressor protein involved in DNA damage response.

Purpose of the Study:

To elucidate the regulatory mechanism between YAP and p73 in response to DNA damage.
To investigate the role of PML in mediating YAP-p73 interactions.

Main Methods:

Co-immunoprecipitation assays to detect protein interactions.
Western blotting to assess protein levels and modifications.
Reporter assays to measure transcriptional activity.

Main Results:

YAP directly binds to p73.

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YAP activation of p73 leads to increased PML transcription.

PML mediates YAP sumoylation and subsequent stabilization.

This creates a positive feedback loop enhancing p73 activity.

Conclusions:

YAP and p73 form a regulatory axis crucial for DNA damage response.
PML acts as a key mediator in this feedback loop, amplifying p73 function.
This mechanism highlights a novel pathway for tumor suppression and cellular protection.