Related Experiment Video
Updated: Jun 26, 2026
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
Enhancement of neutral endopeptidase activity in SK-N-SH cells by ginsenoside Rb1
Ling-Ling Yang1, Shan Duan, Jian-Rong Hao
1Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, Shandong, China.
Objectives:
To investigate the effect of ginsenosides Rb1 and Rg1 on Neprilysin (NEP) activity in SK-N-SH cells, and probe the underlying mechanism.
Methods:
The effects of ginsenosides Rb1 and Rg1 on NEP activity were analyzed by NEP peptidase assay. Western blot was used to determine NEP gene expression at translational level, and RT-PCR was also performed to detect NEP gene expression at transcriptional level.
Results:
NEP peptidase assay indicated that ginsenoside Rb1 can improve the activity of NEP, and RT-PCR and western blot results showed that the enhancement of NEP activity by ginsenoside Rb1 was due to enhancing NEP gene expression, while Rg1 did not have this effect.
Conclusion:
Our studies showed that ginsenoside Rb1 can enhance NEP activity by upregulating NEP gene expression. Our findings might offer a pharmacological explanation for the use of ginseng in traditional medicine.
