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Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

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Related Experiment Video

Updated: Jun 26, 2026

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples
09:29

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples

Published on: April 10, 2026

Human pluripotent stem cells: current status and future perspectives.

Cheng-Fu Kao1, Ching-Yu Chuang, Chien-Hong Chen

  • 1Genomics Research Center, Academia Sinica, Taipei, Taiwan, Republic of China.

The Chinese Journal of Physiology
|December 31, 2008
PubMed
Summary
This summary is machine-generated.

New methods for deriving human pluripotent stem cells (PSCs) avoid embryo destruction, offering ethical alternatives for regenerative medicine. Research explores induced PSCs, single blastomere derivation, and SCNT, alongside epigenetic screening and ChIP-seq advancements.

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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Related Experiment Videos

Last Updated: Jun 26, 2026

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples
09:29

Reprogramming Induced Pluripotent Stem Cell Lines from Frozen Buffy Coat Samples

Published on: April 10, 2026

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
08:00

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Developmental biology

Background:

  • Human embryonic stem cells (hESCs) hold therapeutic promise for degenerative diseases.
  • Conventional hESC derivation methods involve embryo destruction, raising ethical concerns.
  • Alternative methods are crucial for advancing stem cell therapies.

Purpose of the Study:

  • To review novel human pluripotent stem cell (hPSC) derivation methods.
  • To address challenges and ethical considerations in hPSC generation.
  • To discuss the implications of new PSC types for regenerative medicine.

Main Methods:

  • Review of alternative hPSC derivation techniques, including induced PSCs, single blastomere derivation, and SCNT.
  • Examination of pluripotency and reprogramming mechanisms.
  • Summary of genome-wide chromatin immunoprecipitation-sequencing (ChIP-seq) advancements.

Main Results:

  • New PSC derivation methods overcome ethical and technical hurdles of traditional hESC isolation.
  • Advances in induced PSCs, single blastomere derivation, and SCNT offer viable alternatives.
  • Genome-wide epigenetic screening and ChIP-seq provide insights into reprogramming.

Conclusions:

  • Novel hPSC derivation strategies are advancing regenerative medicine.
  • Understanding pluripotency and reprogramming is key to harnessing PSC potential.
  • ChIP-seq technology is vital for stem cell research and therapeutic development.