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Updated: Jun 26, 2026
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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
Vesicle formation and endocytosis: function, machinery, mechanisms, and modeling
Nihal S Parkar1, Belinda S Akpa, Ludwig C Nitsche
1Department of Chemical Engineering, College of Engineering and College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
Antioxidants & Redox Signaling
|December 31, 2008
Summary
Cellular vesicle formation, crucial for nutrient uptake and pathogen entry, involves complex molecular machinery. This review details endocytosis mechanisms, proteins, and their roles in health and disease.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Endocytosis is vital for cellular processes like nutrient uptake, hormone signaling, and pathogen entry.
- Understanding endocytic pathways is key to advancing drug delivery and disease treatment.
- Caveolae- and clathrin-mediated endocytosis, alongside novel pathways, are areas of active research.
Purpose of the Study:
- To provide a comprehensive review of the molecular machinery governing endocytic vesicle formation.
- To discuss the structural and signaling proteins involved in endocytosis.
- To explore the biochemical functions, diseases, and computational modeling of endocytosis.
Main Methods:
- Literature review of structural and signaling proteins in endocytosis.
- Discussion of biochemical functions and disease implications.
- Exploration of computational approaches for simulating endocytosis.
Main Results:
- Detailed overview of proteins and molecular mechanisms driving vesicle budding, invagination, and fission.
- Insights into the recruitment of endocytic machinery to the plasma membrane.
- Identification of cell-type and species-specific variations in endocytic pathways.
Conclusions:
- Endocytosis is a complex, multi-component process essential for cellular function.
- Disruptions in endocytosis are linked to various diseases.
- Further research, including computational modeling, is needed to fully elucidate endocytic mechanisms.
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