High-potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa azurins
Kyle M Lancaster1, Keiko Yokoyama, John H Richards
1Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA.
Inorganic Chemistry
|December 31, 2008
Abstract:
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu(II/I) reduction potentials relative to the Cu(II) aquo ion. The high potential and low axial hyperfine splitting (Cu(II) electron paramagnetic resonance A( parallel)) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.
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