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Potential for La Crosse virus segment reassortment in nature
Sara M Reese1, Bradley J Blitvich, Carol D Blair
1Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1692, USA. hex5@cdc.gov
Virology Journal
|December 31, 2008
Summary
La Crosse virus (LACV) reassorts its genome segments in nature. This genetic exchange in mosquitoes is frequent, contributing to the virus
Area of Science:
- Virology
- Genetics
- Ecology
Background:
- La Crosse virus (LACV) exhibits evolutionary success attributed to genetic adaptability.
- Segment reassortment, a mechanism for genetic change, is facilitated by vertical transmission in mosquitoes.
- Understanding LACV reassortment in natural populations is crucial for predicting viral evolution.
Purpose of the Study:
- To investigate the occurrence and frequency of genome segment reassortment in naturally infected Aedes triseriatus mosquitoes.
- To analyze LACV genetic diversity and reassortment patterns in Wisconsin and Minnesota.
Main Methods:
- Collection of Aedes triseriatus mosquito eggs from endemic areas in Wisconsin and Minnesota.
- Laboratory rearing of mosquitoes and detection of LACV antigen using immunofluorescence assay.
- RNA isolation, RT-PCR amplification of viral genome segments (S, M, L), sequencing, and phylogenetic analysis.
Main Results:
- Analysis of viral sequences from 40 infected mosquitoes and 5 virus isolates.
- Phylogenetic and linkage disequilibrium analyses indicated significant segment reassortment.
- Approximately 25% of infected mosquitoes and viruses harbored reassorted genome segments.
Conclusions:
- Segment reassortment is a frequent event in natural LACV infections.
- The high frequency of reassortment suggests a significant role in LACV evolution and adaptation.
- Findings highlight the importance of genetic exchange in Bunyaviridae family viruses.
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