Related Experiment Video
Updated: Jun 26, 2026
10:34
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
Published on: April 23, 2017
Targeting nanodisks via a single chain variable antibody--apolipoprotein chimera
David M Iovannisci1, Jennifer A Beckstead, Robert O Ryan
1Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
Biochemical and Biophysical Research Communications
|December 31, 2008
Summary
Researchers developed targeted nanodisks (ND) for drug delivery. A novel chimera protein enables these nanodisks to recognize specific antigens, improving drug targeting to tissues.
Area of Science:
- Biotechnology
- Nanomedicine
- Drug Delivery Systems
Background:
- Nanodisks (ND) are nanoscale complexes of lipids and proteins used as drug carriers.
- Current ND formulations lack specific targeting capabilities.
- Previous NDs have successfully carried drugs like amphotericin B and all-trans retinoic acid.
Purpose of the Study:
- To engineer targeted nanodisks for enhanced drug delivery.
- To create a fusion protein combining antibody fragments with apolipoprotein for ND formation and antigen recognition.
Main Methods:
- Construction of a single-chain variable fragment (scFv).apolipoprotein A-I chimera.
- Assessment of the chimera's ability to form functional nanodisks.
- Evaluation of the nanodisks' antigen recognition capabilities.
Main Results:
- The alpha-vimentin scFv.apolipoprotein A-I chimera successfully formed nanodisks.
- The engineered nanodisks demonstrated antigen recognition.
- The fusion protein was functional in both nanodisk formation and specific binding.
Conclusions:
- Engineered scFv.apolipoprotein chimeras can form functional nanodisks.
- This approach enables targeted drug delivery by directing nanodisks to specific tissues.
- This represents a significant advancement in developing targeted nanodisk-based therapeutics.
